diff --git a/mite_data/data/200b1b66-5f29-11f1-bc58-c13514790332.json b/mite_data/data/200b1b66-5f29-11f1-bc58-c13514790332.json new file mode 100644 index 00000000..88ec380c --- /dev/null +++ b/mite_data/data/200b1b66-5f29-11f1-bc58-c13514790332.json @@ -0,0 +1,69 @@ +{ + "accession": "MITE9999999", + "status": "pending", + "comment": "StrB installs the Lys-to-Trp crosslink in the biosynthesis of streptide", + "changelog": [ + { + "version": "1", + "date": "2026-06-03", + "contributors": [ + "0009-0008-8086-5325" + ], + "reviewers": [ + "BBBBBBBBBBBBBBBBBBBBBBBB" + ], + "comment": "New entry." + } + ], + "enzyme": { + "name": "StrB", + "description": "Radical SAM enzyme", + "references": [ + "doi:10.1038/nchem.2237" + ], + "databaseIds": { + "uniprot": "UPI0000E5643D", + "genpept": "ABJ66529.1", + "mibig": "BGC0001209" + }, + "cofactors": { + "organic": [ + "SAM" + ], + "inorganic": [ + "FeS" + ] + } + }, + "reactions": [ + { + "tailoring": [ + "Biaryl bond formation", + "Cyclization" + ], + "description": "StrB installs the Lys-to-Trp crosslink through a C-C bond between the C\u03b2 of Lys and the aromatic C7 of Trp", + "reactionSMARTS": 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+ "reactions": [ + { + "substrate": "CSCC[C@H](NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CCSC)C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)NCC(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)NCC(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCC(=O)O)C(=O)O)C(C)C", + "products": [ + "CSCC[C@H](NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CCSC)C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H]1C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)NCC(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)NCC(=O)N[C@@H](Cc2c[nH]c3ccccc23)C(=O)N[C@@H](CCC(=O)O)C(=O)O)C(C)C)Cc2c[nH]c3c(cccc23)C1CCCN" + ], + "isIntermediate": true, + "description": "The strA product, 30mer precursor peptide is acted upon by StrB to install the Lys-to-Trp crosslink. The cross-linked product is then further processed likely involving StrC and proteases, to give the final 9-mer cyclic streptide." + } + ], + "evidence": { + "evidenceCode": [ + "Heterologous expression", + "In vitro assay", + "Knock-out studies", + "Site-directed mutagenesis" + ], + "references": [ + "doi:10.1038/nchem.2237" + ] + } + } + ] +} \ No newline at end of file