Since we are using RidgeCV to normalize phospho, it is important to point out the vulnerability this approach has when the data is not normally distributed but instead bi-modal or multi-modally. Examples when this can happen is when two tumor types are combined such as lung and breast, or when tumor and normal data is analyzed together. In this example one would normalize phospho separately for lung tumors, lung normals, breast tumors and breast normals.
Since we are using RidgeCV to normalize phospho, it is important to point out the vulnerability this approach has when the data is not normally distributed but instead bi-modal or multi-modally. Examples when this can happen is when two tumor types are combined such as lung and breast, or when tumor and normal data is analyzed together. In this example one would normalize phospho separately for lung tumors, lung normals, breast tumors and breast normals.