mite-standard · mite-bot · Jun 3, 2026 · Jun 3, 2026
diff --git a/mite_data/data/200b1b66-5f29-11f1-bc58-c13514790332.json b/mite_data/data/200b1b66-5f29-11f1-bc58-c13514790332.json
@@ -0,0 +1,69 @@
+{
+    "accession": "MITE9999999",
+    "status": "pending",
+    "comment": "StrB installs the Lys-to-Trp crosslink in the biosynthesis of streptide",
+    "changelog": [
+        {
+            "version": "1",
+            "date": "2026-06-03",
+            "contributors": [
+                "0009-0008-8086-5325"
+            ],
+            "reviewers": [
+                "BBBBBBBBBBBBBBBBBBBBBBBB"
+            ],
+            "comment": "New entry."
+        }
+    ],
+    "enzyme": {
+        "name": "StrB",
+        "description": "Radical SAM enzyme",
+        "references": [
+            "doi:10.1038/nchem.2237"
+        ],
+        "databaseIds": {
+            "uniprot": "UPI0000E5643D",
+            "genpept": "ABJ66529.1",
+            "mibig": "BGC0001209"
+        },
+        "cofactors": {
+            "organic": [
+                "SAM"
+            ],
+            "inorganic": [
+                "FeS"
+            ]
+        }
+    },
+    "reactions": [
+        {
+            "tailoring": [
+                "Biaryl bond formation",
+                "Cyclization"
+            ],
+            "description": "StrB installs the Lys-to-Trp crosslink through a C-C bond between the C\u03b2 of Lys and the aromatic C7 of Trp",
+            "reactionSMARTS": "[#6:1]-[#16:2]-[#6:3]-[#6:4]\\[#6@@:5](-[#6:107](-[#7:109]-[#6@:110](-[#6:112](-[#7:114]\\[#6@:115](-[#6:121](-[#7:123]-[#6:124]-[#6:125](-[#7:127]/[#6@:128](-[#6:133](-[#7:135]-[#6:136]-[#6:137](-[#7:139]\\[#6@:140](-[#6:151](-[#7:153]\\[#6@:154](-[#6:160](-[#7:162]/[#6@@:163](-[#6:228](-[#6:230])-[#6:229])-[#6:164](-[#7:166]\\[#6@:167](-[#6:172](-[#7:174]-[#6@:175](-[#6:177](-[#7:179]\\[#6@:180](-[#6:186](-[#7:188]-[#6:189]-[#6:190](-[#7:192]\\[#6@:193](-[#6:198](-[#7:200]-[#6:201]-[#6:202](-[#7:204]\\[#6@:205](-[#6:216](-[#7:218]\\[#6@:219](-[#6:225](-[#8:227])=[#8:226])-[#6:220]-[#6:221]-[#6:222](-[#8:224])=[#8:223])=[#8:217])-[#6:206]-[#6:207]1:[#6:215]2:[#6:210](:[#6:211]:[#6:212]:[#6:213]:[#6:214]:2):[#7:209]:[#6:208]:1)=[#8:203])=[#8:199])-[#6:194]-[#6:195](-[#8:197])=[#8:196])=[#8:191])=[#8:187])-[#6:181]-[#6:182]-[#6:183]-[#6:184]-[#7:185])=[#8:178])/[#6:176])=[#8:173])-[#6:168]-[#6:169]-[#16:170]-[#6:171])=[#8:165])=[#8:161])-[#6:155]-[#6:156]-[#6:157]-[#6:158]-[#7:159])=[#8:152])-[#6:141]-[#6:142]1:[#6:150]2:[#6:145](:[#6:146]:[#6:147]:[#6:148]:[#6:149]:2):[#7:144]:[#6:143]:1)=[#8:138])=[#8:134])-[#6:129]-[#6:130](-[#8:132])=[#8:131])=[#8:126])=[#8:122])-[#6:116]-[#6:117]-[#6:118]-[#6:119]-[#7:120])=[#8:113])/[#6:111])=[#8:108])-[#7:6]-[#6:7](/[#6@@:9](-[#7:12]-[#6:13](\\[#6@@:15](-[#7:18]-[#6:19](/[#6@@:21](-[#7:24]-[#6:25](\\[#6@@:27](-[#7:33]-[#6:34](/[#6@@:36](-[#7:41]-[#6:42](\\[#6@:44](-[#6:104](-[#6:106])-[#6:105])-[#7:45]-[#6:46](/[#6@@:48](-[#7:54]-[#6:55](\\[#6@@:57](-[#7:63]-[#6:64](/[#6@@:66](-[#7:71]-[#6:72](\\[#6@@:74](-[#7:80]-[#6:81](/[#6@@:83](-[#7:89]-[#6:90](\\[#6@@:92](-[#7:95]-[#6:96](-[#6@:98](-[#6:100]-[#6:101]-[#16:102]-[#6:103])\\[#7:99])=[#8:97])-[#6:93]-[#8:94])=[#8:91])-[#6:84]-[#6:85]-[#6:86]-[#6:87]-[#7:88])=[#8:82])-[#6:75]-[#6:76]-[#6:77](-[#8:79])=[#8:78])=[#8:73])-[#6:67]-[#6:68](-[#6:70])-[#6:69])=[#8:65])-[#6:58]-[#6:59]-[#6:60](-[#8:62])=[#8:61])=[#8:56])-[#6:49]-[#6:50]-[#6:51]-[#6:52]-[#7:53])=[#8:47])=[#8:43])-[#6:37]-[#6:38](-[#6:40])-[#6:39])=[#8:35])-[#6:28]-[#6:29]-[#6:30](-[#8:32])=[#8:31])=[#8:26])-[#6:22]-[#8:23])=[#8:20])-[#6:16]-[#8:17])=[#8:14])-[#6:10]-[#8:11])=[#8:8]>>[#6:1]-[#16:2]-[#6:3]-[#6:4]\\[#6@@:5](-[#6:107](-[#7:109]-[#6@:110](-[#6:112](-[#7:114]\\[#6@@:115]1-[#6:121](-[#7:123]-[#6:124]-[#6:125](-[#7:127]/[#6@:128](-[#6:133](-[#7:135]-[#6:136]-[#6:137](-[#7:139]\\[#6@:140](-[#6:151](-[#7:153]\\[#6@:154](-[#6:160](-[#7:162]/[#6@@:163](-[#6:228](-[#6:230])-[#6:229])-[#6:164](-[#7:166]\\[#6@:167](-[#6:172](-[#7:174]-[#6@:175](-[#6:177](-[#7:179]\\[#6@:180](-[#6:186](-[#7:188]-[#6:189]-[#6:190](-[#7:192]\\[#6@:193](-[#6:198](-[#7:200]-[#6:201]-[#6:202](-[#7:204]\\[#6@:205](-[#6:216](-[#7:218]\\[#6@:219](-[#6:225](-[#8:227])=[#8:226])-[#6:220]-[#6:221]-[#6:222](-[#8:224])=[#8:223])=[#8:217])-[#6:206]-[#6:207]2:[#6:215]3:[#6:210](:[#6:211]:[#6:212]:[#6:213]:[#6:214]:3):[#7:209]:[#6:208]:2)=[#8:203])=[#8:199])-[#6:194]-[#6:195](-[#8:197])=[#8:196])=[#8:191])=[#8:187])-[#6:181]-[#6:182]-[#6:183]-[#6:184]-[#7:185])=[#8:178])/[#6:176])=[#8:173])-[#6:168]-[#6:169]-[#16:170]-[#6:171])=[#8:165])=[#8:161])-[#6:155]-[#6:156]-[#6:157]-[#6:158]-[#7:159])=[#8:152])-[#6:141]-[#6:142]2:[#6:150]3:[#6:145](:[#6:146](-[#6:116]-1-[#6:117]-[#6:118]-[#6:119]-[#7:120]):[#6:147]:[#6:148]:[#6:149]:3):[#7:144]:[#6:143]:2)=[#8:138])=[#8:134])-[#6:129]-[#6:130](-[#8:132])=[#8:131])=[#8:126])=[#8:122])=[#8:113])/[#6:111])=[#8:108])-[#7:6]-[#6:7](/[#6@@:9](-[#7:12]-[#6:13](\\[#6@@:15](-[#7:18]-[#6:19](/[#6@@:21](-[#7:24]-[#6:25](\\[#6@@:27](-[#7:33]-[#6:34](/[#6@@:36](-[#7:41]-[#6:42](\\[#6@:44](-[#6:104](-[#6:105])-[#6:106])-[#7:45]-[#6:46](/[#6@@:48](-[#7:54]-[#6:55](\\[#6@@:57](-[#7:63]-[#6:64](/[#6@@:66](-[#7:71]-[#6:72](\\[#6@@:74](-[#7:80]-[#6:81](/[#6@@:83](-[#7:89]-[#6:90](\\[#6@@:92](-[#7:95]-[#6:96](-[#6@:98](-[#6:100]-[#6:101]-[#16:102]-[#6:103])\\[#7:99])=[#8:97])-[#6:93]-[#8:94])=[#8:91])-[#6:84]-[#6:85]-[#6:86]-[#6:87]-[#7:88])=[#8:82])-[#6:75]-[#6:76]-[#6:77](-[#8:79])=[#8:78])=[#8:73])-[#6:67]-[#6:68](-[#6:69])-[#6:70])=[#8:65])-[#6:58]-[#6:59]-[#6:60](-[#8:62])=[#8:61])=[#8:56])-[#6:49]-[#6:50]-[#6:51]-[#6:52]-[#7:53])=[#8:47])=[#8:43])-[#6:37]-[#6:38](-[#6:40])-[#6:39])=[#8:35])-[#6:28]-[#6:29]-[#6:30](-[#8:32])=[#8:31])=[#8:26])-[#6:22]-[#8:23])=[#8:20])-[#6:16]-[#8:17])=[#8:14])-[#6:10]-[#8:11])=[#8:8]",
+            "reactions": [
+                {
+                    "substrate": "CSCC[C@H](NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CCSC)C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)NCC(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)NCC(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCC(=O)O)C(=O)O)C(C)C",
+                    "products": [
+                        "CSCC[C@H](NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CCSC)C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H]1C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)NCC(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)NCC(=O)N[C@@H](Cc2c[nH]c3ccccc23)C(=O)N[C@@H](CCC(=O)O)C(=O)O)C(C)C)Cc2c[nH]c3c(cccc23)C1CCCN"
+                    ],
+                    "isIntermediate": true,
+                    "description": "The strA product, 30mer precursor peptide is acted upon by StrB to install the Lys-to-Trp crosslink. The cross-linked product is then further processed likely involving StrC and proteases, to give the final 9-mer cyclic streptide."
+                }
+            ],
+            "evidence": {
+                "evidenceCode": [
+                    "Heterologous expression",
+                    "In vitro assay",
+                    "Knock-out studies",
+                    "Site-directed mutagenesis"
+                ],
+                "references": [
+                    "doi:10.1038/nchem.2237"
+                ]
+            }
+        }
+    ]
+}